intracellular staining kit ebioscience 00-5523-00  (Thermo Fisher)

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Brown fat-specific mitoribosomal function is crucial for preventing cold exposure-induced bone loss

doi: 10.1007/s00018-024-05347-4

Figure Lengend Snippet: Temperature-mediated changes in osteoclastogenic bone marrow T cells and brown adipose tissue (BAT). A Representative contour plots from flow cytometry for regulatory T-cells (Treg; CD4 + CD25 + FOXP3+); RANKL (CD254) producing Treg cells; and IFN-γ or IL-17 A-producing CD4 + cells from bone marrow. B Statistical analysis of phenotypes defined by flow cytometry. C , D Representative western blots and band density measurements of UCP-1 and OxPhos complex subunits in the BAT isolated from mice housed for 12 weeks at 22–30 °C. E Representative immunohistochemical images of UCP-1 and SDH staining of BAT from mice housed at different temperatures. Scale bar, 50 μm. Data were expressed as the mean ± SD. Statistical significance was estimated using unpaired t-tests. *, P < 0.05, **, P < 0.01 ***, P < 0.001

Article Snippet: Foxp3 staining was performed after fixation and permeabilization using the eBioscience Foxp3 staining kit.

Techniques: Flow Cytometry, Western Blot, Isolation, Immunohistochemical staining, Staining

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Brown fat-specific mitoribosomal function is crucial for preventing cold exposure-induced bone loss

doi: 10.1007/s00018-024-05347-4

Figure Lengend Snippet: BAT-specific mitochondrial dysfunction (BKO) mice exposed to cold stress exhibit bone loss and an osteoclastogenic bone marrow T-cell phenotype. A Micro-CT scans showing the cortical and trabecular structures in the distal part of the femur. B Measurement of Tb.BMD, Tb.BV/TV, Tb.Th, Tb.N, Tb.Sp, Ct.BMD, Ct.BV and Ct.Th in the femur. C Representative contour plots from flow cytometry for regulatory T-cells (Treg; CD4 + CD25 + FOXP3+); RANKL (CD254) producing Treg cells; and IFN-γ producing CD4 + cells from bone marrow. D Serum levels of RANKL. Data are expressed as the mean ± SD. Statistical significance was determined using one-way ANOVA for comparative analysis. *, P < 0.05 and **, P < 0.01 ***, P < 0.001

Article Snippet: Foxp3 staining was performed after fixation and permeabilization using the eBioscience Foxp3 staining kit.

Techniques: Micro-CT, Flow Cytometry

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Brown fat-specific mitoribosomal function is crucial for preventing cold exposure-induced bone loss

doi: 10.1007/s00018-024-05347-4

Figure Lengend Snippet: Treatment with palmitic acid increases the number of osteoclasts and promotes production of RANKL and IFN-γ in bone marrow T cells. A TRAP staining of osteoclasts and the number of TRAP-positive osteoclasts. Scale bar: 200 μm. B RANKL producing cells in Foxp3 + Treg populations treated with or without palmitic acid. C IFN-γ producing cells within the CD4 + T-cell populations treated with or without palmitic acid. Statistical significance was analyzed by one-way ANOVA. *, P < 0.05 and **, P < 0.01 compared with the indicated group

Article Snippet: Foxp3 staining was performed after fixation and permeabilization using the eBioscience Foxp3 staining kit.

Techniques: Staining